Construct: shRNA TRCN0000255118

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033820.3-2117s21c1
DNA Barcode:
TGCTGCCTAGCGTGTTCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp551 (619331)
Gene Description:
zinc fingr protein 551
Transcript:
RefSeq NM_001033820.3 (CURRENT)
Match location:
Position 2117 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255118)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGCCTAGCGTGTTCTTTA
Hairpin Sequence:
5'-CCGG-TGCTGCCTAGCGTGTTCTTTA-CTCGAG-TAAAGAACACGCTAGGCAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGCCTAGCGTGTTCTTTACTCGAGTAAAGAACACGCTAGGCAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGCCTAGCGTGTTCTTTACTCGAGTAAAGAACACGCTAGGCAGCA-3'

Other clones with same target sequence:

(none)