Construct: shRNA TRCN0000255140

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133237.3-1537s21c1
DNA Barcode:
CCAACAGGATGTGACACATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Apcdd1 (494504)
Gene Description:
adenomatosis polyposis coli down-regulated 1
Transcript:
RefSeq NM_133237.3 (CURRENT)
Match location:
Position 1537 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255140)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACAGGATGTGACACATAC
Hairpin Sequence:
5'-CCGG-CCAACAGGATGTGACACATAC-CTCGAG-GTATGTGTCACATCCTGTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACAGGATGTGACACATACCTCGAGGTATGTGTCACATCCTGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACAGGATGTGACACATACCTCGAGGTATGTGTCACATCCTGTTGG-3'

Other clones with same target sequence:

(none)