Construct: shRNA TRCN0000255160

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039242.2-440s21c1
DNA Barcode:
TCAGAATATCAGGCTTGTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
March10 (632687)
Gene Description:
membrane-associated ring finger (C3HC4) 10
Transcript:
RefSeq NM_001039242.2 (CURRENT)
Match location:
Position 440 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255160)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAGAATATCAGGCTTGTTTG
Hairpin Sequence:
5'-CCGG-TCAGAATATCAGGCTTGTTTG-CTCGAG-CAAACAAGCCTGATATTCTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAGAATATCAGGCTTGTTTGCTCGAGCAAACAAGCCTGATATTCTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAGAATATCAGGCTTGTTTGCTCGAGCAAACAAGCCTGATATTCTGA-3'

Other clones with same target sequence:

(none)