Construct: shRNA TRCN0000255180

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081283.1-551s21c1
DNA Barcode:
ACTTGCTCAAAGGCCACTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem28 (620592)
Gene Description:
transmembrane protein 28
Transcript:
RefSeq NM_001081283.1 (CURRENT)
Match location:
Position 551 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255180)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTGCTCAAAGGCCACTTTC
Hairpin Sequence:
5'-CCGG-ACTTGCTCAAAGGCCACTTTC-CTCGAG-GAAAGTGGCCTTTGAGCAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTGCTCAAAGGCCACTTTCCTCGAGGAAAGTGGCCTTTGAGCAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTGCTCAAAGGCCACTTTCCTCGAGGAAAGTGGCCTTTGAGCAAGT-3'

Other clones with same target sequence:

(none)