Construct: shRNA TRCN0000255190

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001099298.2-5829s21c1
DNA Barcode:
TAAGAAGGTTTCGTCTATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scn2a (110876)
Gene Description:
sodium channel, voltage-gated, type II, alpha
Transcript:
RefSeq NM_001099298.2 (NON-CURRENT)
Match location:
Position 5829 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255190)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAGAAGGTTTCGTCTATATA
Hairpin Sequence:
5'-CCGG-TAAGAAGGTTTCGTCTATATA-CTCGAG-TATATAGACGAAACCTTCTTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAGAAGGTTTCGTCTATATACTCGAGTATATAGACGAAACCTTCTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAGAAGGTTTCGTCTATATACTCGAGTATATAGACGAAACCTTCTTA-3'

Other clones with same target sequence:

(none)