Construct: shRNA TRCN0000255217

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001042503.1-4641s21c1
DNA Barcode:
TTTGCTAAGAAACTCGATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trim71 (636931)
Gene Description:
tripartite motif-containing 71
Transcript:
RefSeq NM_001042503.1 (NON-CURRENT)
Match location:
Position 4641 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255217)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTGCTAAGAAACTCGATTTA
Hairpin Sequence:
5'-CCGG-TTTGCTAAGAAACTCGATTTA-CTCGAG-TAAATCGAGTTTCTTAGCAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTGCTAAGAAACTCGATTTACTCGAGTAAATCGAGTTTCTTAGCAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTGCTAAGAAACTCGATTTACTCGAGTAAATCGAGTTTCTTAGCAAA-3'

Other clones with same target sequence:

(none)