Construct: shRNA TRCN0000255287

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030746.1-1325s21c1
DNA Barcode:
ACACTGGGACAGCTTTATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Btnl4 (632126)
Gene Description:
butyrophilin-like 4
Transcript:
RefSeq NM_030746.1 (CURRENT)
Match location:
Position 1325 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255287)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACTGGGACAGCTTTATTAT
Hairpin Sequence:
5'-CCGG-ACACTGGGACAGCTTTATTAT-CTCGAG-ATAATAAAGCTGTCCCAGTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACTGGGACAGCTTTATTATCTCGAGATAATAAAGCTGTCCCAGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACTGGGACAGCTTTATTATCTCGAGATAATAAAGCTGTCCCAGTGT-3'

Other clones with same target sequence:

(none)