Construct: shRNA TRCN0000255290

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001037746.2-1390s21c1
DNA Barcode:
TAGGATGCCCTACCTGCATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prps1l3 (328099)
Gene Description:
phosphoribosyl pyrophosphate synthetase 1-like 3
Transcript:
RefSeq NM_001037746.2 (NON-CURRENT)
Match location:
Position 1390 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255290)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGGATGCCCTACCTGCATTG
Hairpin Sequence:
5'-CCGG-TAGGATGCCCTACCTGCATTG-CTCGAG-CAATGCAGGTAGGGCATCCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGGATGCCCTACCTGCATTGCTCGAGCAATGCAGGTAGGGCATCCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGGATGCCCTACCTGCATTGCTCGAGCAATGCAGGTAGGGCATCCTA-3'

Other clones with same target sequence:

(none)