Construct: shRNA TRCN0000255305

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001101038.1-455s21c1
DNA Barcode:
ATCGCTATGAGAGTCGCCATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Siglec15 (620235)
Gene Description:
sialic acid binding Ig-like lectin 15
Transcript:
RefSeq NM_001101038.1 (CURRENT)
Match location:
Position 455 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255305)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCGCTATGAGAGTCGCCATG
Hairpin Sequence:
5'-CCGG-ATCGCTATGAGAGTCGCCATG-CTCGAG-CATGGCGACTCTCATAGCGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCGCTATGAGAGTCGCCATGCTCGAGCATGGCGACTCTCATAGCGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCGCTATGAGAGTCGCCATGCTCGAGCATGGCGACTCTCATAGCGAT-3'

Other clones with same target sequence:

(none)