Construct: shRNA TRCN0000255312

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153160.3-571s21c1
DNA Barcode:
GCCAGGTGGAACCAAGTATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zcchc17 (619605)
Gene Description:
zinc finger, CCHC domain containing 17
Transcript:
RefSeq NM_153160.3 (NON-CURRENT)
Match location:
Position 571 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255312)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGGTGGAACCAAGTATTC
Hairpin Sequence:
5'-CCGG-GCCAGGTGGAACCAAGTATTC-CTCGAG-GAATACTTGGTTCCACCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGGTGGAACCAAGTATTCCTCGAGGAATACTTGGTTCCACCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGGTGGAACCAAGTATTCCTCGAGGAATACTTGGTTCCACCTGGC-3'

Other clones with same target sequence:

(none)