Construct: shRNA TRCN0000255323

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001114754.1-242s21c1
DNA Barcode:
CATTTGGCAAACATGAGAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm6121 (619991)
Gene Description:
predicted gene 6121
Transcript:
RefSeq NM_001114754.1 (CURRENT)
Match location:
Position 242 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255323)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTTGGCAAACATGAGAATA
Hairpin Sequence:
5'-CCGG-CATTTGGCAAACATGAGAATA-CTCGAG-TATTCTCATGTTTGCCAAATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTTGGCAAACATGAGAATACTCGAGTATTCTCATGTTTGCCAAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTTGGCAAACATGAGAATACTCGAGTATTCTCATGTTTGCCAAATG-3'

Other clones with same target sequence:

(none)