Construct: shRNA TRCN0000255404

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080432.1-505s21c1
DNA Barcode:
TCTCGCATCCTCATTGGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FTO (79068)
Gene Description:
FTO alpha-ketoglutarate dependent dioxygenase
Transcript:
RefSeq NM_001080432.1 (NON-CURRENT)
Match location:
Position 505 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255404)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTCGCATCCTCATTGGTAAT
Hairpin Sequence:
5'-CCGG-TCTCGCATCCTCATTGGTAAT-CTCGAG-ATTACCAATGAGGATGCGAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTCGCATCCTCATTGGTAATCTCGAGATTACCAATGAGGATGCGAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTCGCATCCTCATTGGTAATCTCGAGATTACCAATGAGGATGCGAGA-3'

Other clones with same target sequence:

(none)