Construct: shRNA TRCN0000255407

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000043.3-1984s21c1
DNA Barcode:
TTAAATTATAATGTTTGACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAS (355)
Gene Description:
Fas cell surface death receptor
Transcript:
RefSeq NM_000043.3 (NON-CURRENT)
Match location:
Position 1984 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255407)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTAAATTATAATGTTTGACTA
Hairpin Sequence:
5'-CCGG-TTAAATTATAATGTTTGACTA-CTCGAG-TAGTCAAACATTATAATTTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTAAATTATAATGTTTGACTACTCGAGTAGTCAAACATTATAATTTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTAAATTATAATGTTTGACTACTCGAGTAGTCAAACATTATAATTTAA-3'

Other clones with same target sequence:

(none)