Construct: shRNA TRCN0000255475

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004799.2-1370s21c1
DNA Barcode:
ACTAATTGGAAGTTGACTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZFYVE9 (9372)
Gene Description:
zinc finger FYVE-type containing 9
Transcript:
RefSeq NM_004799.2 (NON-CURRENT)
Match location:
Position 1370 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255475)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTAATTGGAAGTTGACTAAA
Hairpin Sequence:
5'-CCGG-ACTAATTGGAAGTTGACTAAA-CTCGAG-TTTAGTCAACTTCCAATTAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTAATTGGAAGTTGACTAAACTCGAGTTTAGTCAACTTCCAATTAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTAATTGGAAGTTGACTAAACTCGAGTTTAGTCAACTTCCAATTAGT-3'

Other clones with same target sequence:

(none)