Construct: shRNA TRCN0000255499

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027879.1-1821s21c1
DNA Barcode:
TTTCACTGTCTGGGATCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdc40 (71713)
Gene Description:
cell division cycle 40
Transcript:
RefSeq NM_027879.1 (NON-CURRENT)
Match location:
Position 1821 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255499)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTCACTGTCTGGGATCATTT
Hairpin Sequence:
5'-CCGG-TTTCACTGTCTGGGATCATTT-CTCGAG-AAATGATCCCAGACAGTGAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTCACTGTCTGGGATCATTTCTCGAGAAATGATCCCAGACAGTGAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTCACTGTCTGGGATCATTTCTCGAGAAATGATCCCAGACAGTGAAA-3'

Other clones with same target sequence:

(none)