Construct: shRNA TRCN0000255529

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039042.2-213s21c1
DNA Barcode:
CTTGGCCTTGTCCGAAGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klk13 (626834)
Gene Description:
kallikrein related-peptidase 13
Transcript:
RefSeq NM_001039042.2 (CURRENT)
Match location:
Position 213 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255529)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGGCCTTGTCCGAAGGAAT
Hairpin Sequence:
5'-CCGG-CTTGGCCTTGTCCGAAGGAAT-CTCGAG-ATTCCTTCGGACAAGGCCAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGGCCTTGTCCGAAGGAATCTCGAGATTCCTTCGGACAAGGCCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGGCCTTGTCCGAAGGAATCTCGAGATTCCTTCGGACAAGGCCAAG-3'

Other clones with same target sequence:

(none)