Construct: shRNA TRCN0000255534

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001109970.1-464s21c1
DNA Barcode:
GCTTCAGAATTGGACCTTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm10486 (100039550)
Gene Description:
predicted gene 10486
Transcript:
RefSeq NM_001109970.1 (CURRENT)
Match location:
Position 464 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255534)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCAGAATTGGACCTTATG
Hairpin Sequence:
5'-CCGG-GCTTCAGAATTGGACCTTATG-CTCGAG-CATAAGGTCCAATTCTGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCAGAATTGGACCTTATGCTCGAGCATAAGGTCCAATTCTGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCAGAATTGGACCTTATGCTCGAGCATAAGGTCCAATTCTGAAGC-3'

Other clones with same target sequence:

(none)