Construct: shRNA TRCN0000255545

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001099332.1-1741s21c1
DNA Barcode:
GGAGCCTTTCATCGGCTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cd101 (630146)
Gene Description:
CD101 antigen
Transcript:
RefSeq NM_001099332.1 (NON-CURRENT)
Match location:
Position 1741 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255545)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAGCCTTTCATCGGCTTATT
Hairpin Sequence:
5'-CCGG-GGAGCCTTTCATCGGCTTATT-CTCGAG-AATAAGCCGATGAAAGGCTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAGCCTTTCATCGGCTTATTCTCGAGAATAAGCCGATGAAAGGCTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAGCCTTTCATCGGCTTATTCTCGAGAATAAGCCGATGAAAGGCTCC-3'

Other clones with same target sequence:

(none)