Construct: shRNA TRCN0000255550
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001110217.1-328s21c1
- DNA Barcode:
- CAAAGGCCCTGGTAGCTTATT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Rps16-ps2 (100039355)
- Gene Description:
- ribosomal protein S16, pseudogene 2
- Transcript:
- RefSeq NM_001110217.1 (NON-CURRENT)
- Match location:
- Position 328 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000255550)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CAAAGGCCCTGGTAGCTTATT
- Hairpin Sequence:
-
5'-CCGG-CAAAGGCCCTGGTAGCTTATT-CTCGAG-AATAAGCTACCAGGGCCTTTG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCAAAGGCCCTGGTAGCTTATTCTCGAGAATAAGCTACCAGGGCCTTTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACAAAGGCCCTGGTAGCTTATTCTCGAGAATAAGCTACCAGGGCCTTTG-3'
Other clones with same target sequence:
(none)
