Construct: shRNA TRCN0000255629

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006945.4-260s21c1
DNA Barcode:
GTCCACCCAAGAGCAAGTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPRR2D (6703)
Gene Description:
small proline rich protein 2D
Transcript:
RefSeq NM_006945.4 (NON-CURRENT)
Match location:
Position 260 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255629)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCACCCAAGAGCAAGTAAC
Hairpin Sequence:
5'-CCGG-GTCCACCCAAGAGCAAGTAAC-CTCGAG-GTTACTTGCTCTTGGGTGGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCACCCAAGAGCAAGTAACCTCGAGGTTACTTGCTCTTGGGTGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCACCCAAGAGCAAGTAACCTCGAGGTTACTTGCTCTTGGGTGGAC-3'

Other clones with same target sequence:

(none)