Construct: shRNA TRCN0000255630

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006945.4-203s21c1
DNA Barcode:
AGCAGTGCCAGCAGAAATATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPRR2D (6703)
Gene Description:
small proline rich protein 2D
Transcript:
RefSeq NM_006945.4 (NON-CURRENT)
Match location:
Position 203 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255630)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAGTGCCAGCAGAAATATC
Hairpin Sequence:
5'-CCGG-AGCAGTGCCAGCAGAAATATC-CTCGAG-GATATTTCTGCTGGCACTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAGTGCCAGCAGAAATATCCTCGAGGATATTTCTGCTGGCACTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAGTGCCAGCAGAAATATCCTCGAGGATATTTCTGCTGGCACTGCT-3'

Other clones with same target sequence:

(none)