Construct: shRNA TRCN0000255676

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001098729.1-1447s21c1
DNA Barcode:
TCTTTAGAAGAAGCTTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GTF2H2B (653238)
Gene Description:
general transcription factor IIH subunit 2B (pseudogene)
Transcript:
RefSeq NM_001098729.1 (NON-CURRENT)
Match location:
Position 1447 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255676)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTTAGAAGAAGCTTCATTT
Hairpin Sequence:
5'-CCGG-TCTTTAGAAGAAGCTTCATTT-CTCGAG-AAATGAAGCTTCTTCTAAAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTTAGAAGAAGCTTCATTTCTCGAGAAATGAAGCTTCTTCTAAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTTAGAAGAAGCTTCATTTCTCGAGAAATGAAGCTTCTTCTAAAGA-3'

Other clones with same target sequence:

(none)