Construct: shRNA TRCN0000255747
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_010101.3-3242s21c1
- DNA Barcode:
- GGGTCTAGGTCACTGATATTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- S1pr3 (13610)
- Gene Description:
- sphingosine-1-phosphate receptor 3
- Transcript:
- RefSeq NM_010101.3 (NON-CURRENT)
- Match location:
- Position 3242 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000255747)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GGGTCTAGGTCACTGATATTA
- Hairpin Sequence:
-
5'-CCGG-GGGTCTAGGTCACTGATATTA-CTCGAG-TAATATCAGTGACCTAGACCC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGGGTCTAGGTCACTGATATTACTCGAGTAATATCAGTGACCTAGACCCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGGGTCTAGGTCACTGATATTACTCGAGTAATATCAGTGACCTAGACCC-3'
Other clones with same target sequence:
(none)
