Construct: shRNA TRCN0000255761

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025446.3-625s21c1
DNA Barcode:
TACCCTTTCCTGGAACATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aig1 (66253)
Gene Description:
androgen-induced 1
Transcript:
RefSeq NM_025446.3 (NON-CURRENT)
Match location:
Position 625 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255761)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACCCTTTCCTGGAACATATT
Hairpin Sequence:
5'-CCGG-TACCCTTTCCTGGAACATATT-CTCGAG-AATATGTTCCAGGAAAGGGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACCCTTTCCTGGAACATATTCTCGAGAATATGTTCCAGGAAAGGGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACCCTTTCCTGGAACATATTCTCGAGAATATGTTCCAGGAAAGGGTA-3'

Other clones with same target sequence:

(none)