Construct: shRNA TRCN0000255772

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081257.1-586s21c1
DNA Barcode:
ACTTACAGTAACCTCATATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hpse2 (545291)
Gene Description:
heparanase 2
Transcript:
RefSeq NM_001081257.1 (NON-CURRENT)
Match location:
Position 586 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255772)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTACAGTAACCTCATATTA
Hairpin Sequence:
5'-CCGG-ACTTACAGTAACCTCATATTA-CTCGAG-TAATATGAGGTTACTGTAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTACAGTAACCTCATATTACTCGAGTAATATGAGGTTACTGTAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTACAGTAACCTCATATTACTCGAGTAATATGAGGTTACTGTAAGT-3'

Other clones with same target sequence:

(none)