Construct: shRNA TRCN0000255810

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001102663.1-1522s21c1
DNA Barcode:
ATTCGACTCCTTCAGATTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NBPF16 (728936)
Gene Description:
neuroblastoma breakpoint family, member 16
Transcript:
RefSeq NM_001102663.1 (NON-CURRENT)
Match location:
Position 1522 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255810)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTCGACTCCTTCAGATTATC
Hairpin Sequence:
5'-CCGG-ATTCGACTCCTTCAGATTATC-CTCGAG-GATAATCTGAAGGAGTCGAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTCGACTCCTTCAGATTATCCTCGAGGATAATCTGAAGGAGTCGAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTCGACTCCTTCAGATTATCCTCGAGGATAATCTGAAGGAGTCGAAT-3'

Other clones with same target sequence:

(none)