Construct: shRNA TRCN0000255862

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001135789.1-572s21c1
DNA Barcode:
TCCTGCTTTCACAGAATTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPIAL4C (653598)
Gene Description:
peptidylprolyl isomerase A like 4C
Transcript:
RefSeq NM_001135789.1 (NON-CURRENT)
Match location:
Position 572 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255862)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTGCTTTCACAGAATTATT
Hairpin Sequence:
5'-CCGG-TCCTGCTTTCACAGAATTATT-CTCGAG-AATAATTCTGTGAAAGCAGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTGCTTTCACAGAATTATTCTCGAGAATAATTCTGTGAAAGCAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTGCTTTCACAGAATTATTCTCGAGAATAATTCTGTGAAAGCAGGA-3'

Other clones with same target sequence:

(none)