Construct: shRNA TRCN0000255904

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033176.1-41s21c1
DNA Barcode:
TTCTCCGTGTCCGACATCCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NKX2-4 (644524)
Gene Description:
NK2 homeobox 4
Transcript:
RefSeq NM_033176.1 (NON-CURRENT)
Match location:
Position 41 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255904)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCTCCGTGTCCGACATCCTG
Hairpin Sequence:
5'-CCGG-TTCTCCGTGTCCGACATCCTG-CTCGAG-CAGGATGTCGGACACGGAGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCTCCGTGTCCGACATCCTGCTCGAGCAGGATGTCGGACACGGAGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCTCCGTGTCCGACATCCTGCTCGAGCAGGATGTCGGACACGGAGAA-3'

Other clones with same target sequence:

(none)