Construct: shRNA TRCN0000255953

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030690.2-4645s21c1
DNA Barcode:
AGGATTTGCCACGGGACTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rai14 (75646)
Gene Description:
retinoic acid induced 14
Transcript:
RefSeq NM_030690.2 (NON-CURRENT)
Match location:
Position 4645 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255953)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGATTTGCCACGGGACTTAA
Hairpin Sequence:
5'-CCGG-AGGATTTGCCACGGGACTTAA-CTCGAG-TTAAGTCCCGTGGCAAATCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGATTTGCCACGGGACTTAACTCGAGTTAAGTCCCGTGGCAAATCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGATTTGCCACGGGACTTAACTCGAGTTAAGTCCCGTGGCAAATCCT-3'

Other clones with same target sequence:

(none)