Construct: shRNA TRCN0000256002

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025839.3-108s21c1
DNA Barcode:
TTCATGCCACGCCATGCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nudt16l1 (66911)
Gene Description:
nudix (nucleoside diphosphate linked moiety X)-type motif 16-like 1
Transcript:
RefSeq NM_025839.3 (NON-CURRENT)
Match location:
Position 108 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256002)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCATGCCACGCCATGCTATA
Hairpin Sequence:
5'-CCGG-TTCATGCCACGCCATGCTATA-CTCGAG-TATAGCATGGCGTGGCATGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCATGCCACGCCATGCTATACTCGAGTATAGCATGGCGTGGCATGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCATGCCACGCCATGCTATACTCGAGTATAGCATGGCGTGGCATGAA-3'

Other clones with same target sequence:

(none)