Construct: shRNA TRCN0000256059

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001037161.1-752s21c1
DNA Barcode:
TGATGGCTCTGGCTTACTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACOT1 (641371)
Gene Description:
acyl-CoA thioesterase 1
Transcript:
RefSeq NM_001037161.1 (NON-CURRENT)
Match location:
Position 752 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256059)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATGGCTCTGGCTTACTATA
Hairpin Sequence:
5'-CCGG-TGATGGCTCTGGCTTACTATA-CTCGAG-TATAGTAAGCCAGAGCCATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATGGCTCTGGCTTACTATACTCGAGTATAGTAAGCCAGAGCCATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATGGCTCTGGCTTACTATACTCGAGTATAGTAAGCCAGAGCCATCA-3'

Other clones with same target sequence:

(none)