Construct: shRNA TRCN0000256100

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001098845.1-1437s21c1
DNA Barcode:
GTCTCCTGGGCACAGGTTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANXA8L1 (728113)
Gene Description:
annexin A8 like 1
Transcript:
RefSeq NM_001098845.1 (NON-CURRENT)
Match location:
Position 1437 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256100)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTCCTGGGCACAGGTTATA
Hairpin Sequence:
5'-CCGG-GTCTCCTGGGCACAGGTTATA-CTCGAG-TATAACCTGTGCCCAGGAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTCCTGGGCACAGGTTATACTCGAGTATAACCTGTGCCCAGGAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTCCTGGGCACAGGTTATACTCGAGTATAACCTGTGCCCAGGAGAC-3'

Other clones with same target sequence:

(none)