Construct: shRNA TRCN0000256106

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001042524.1-1334s21c1
DNA Barcode:
AGCTTGCCGCATCTCTAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FRMPD2B (728798)
Gene Description:
FERM and PDZ domain containing 2B, pseudogene
Transcript:
RefSeq NM_001042524.1 (NON-CURRENT)
Match location:
Position 1334 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256106)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTTGCCGCATCTCTAAATA
Hairpin Sequence:
5'-CCGG-AGCTTGCCGCATCTCTAAATA-CTCGAG-TATTTAGAGATGCGGCAAGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTTGCCGCATCTCTAAATACTCGAGTATTTAGAGATGCGGCAAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTTGCCGCATCTCTAAATACTCGAGTATTTAGAGATGCGGCAAGCT-3'

Other clones with same target sequence:

(none)