Construct: shRNA TRCN0000256113

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017774.2-1243s21c1
DNA Barcode:
ACTATTGCTACAGATATTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKAL1 (54901)
Gene Description:
CDK5 regulatory subunit associated protein 1 like 1
Transcript:
RefSeq NM_017774.2 (NON-CURRENT)
Match location:
Position 1243 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256113)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTATTGCTACAGATATTATC
Hairpin Sequence:
5'-CCGG-ACTATTGCTACAGATATTATC-CTCGAG-GATAATATCTGTAGCAATAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTATTGCTACAGATATTATCCTCGAGGATAATATCTGTAGCAATAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTATTGCTACAGATATTATCCTCGAGGATAATATCTGTAGCAATAGT-3'

Other clones with same target sequence:

(none)