Construct: shRNA TRCN0000256216

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001082975.1-656s21c1
DNA Barcode:
ACGTCCAAGGAGTCCTGAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sdr39u1 (654795)
Gene Description:
short chain dehydrogenase/reductase family 39U, member 1
Transcript:
RefSeq NM_001082975.1 (CURRENT)
Match location:
Position 656 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256216)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTCCAAGGAGTCCTGAATG
Hairpin Sequence:
5'-CCGG-ACGTCCAAGGAGTCCTGAATG-CTCGAG-CATTCAGGACTCCTTGGACGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTCCAAGGAGTCCTGAATGCTCGAGCATTCAGGACTCCTTGGACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTCCAAGGAGTCCTGAATGCTCGAGCATTCAGGACTCCTTGGACGT-3'

Other clones with same target sequence:

(none)