Construct: shRNA TRCN0000256240

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001097621.1-1241s21c1
DNA Barcode:
TTCGGAAAGGTGAAGGTTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kif26a (668303)
Gene Description:
kinesin family member 26A
Transcript:
RefSeq NM_001097621.1 (CURRENT)
Match location:
Position 1241 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256240)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCGGAAAGGTGAAGGTTATG
Hairpin Sequence:
5'-CCGG-TTCGGAAAGGTGAAGGTTATG-CTCGAG-CATAACCTTCACCTTTCCGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCGGAAAGGTGAAGGTTATGCTCGAGCATAACCTTCACCTTTCCGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCGGAAAGGTGAAGGTTATGCTCGAGCATAACCTTCACCTTTCCGAA-3'

Other clones with same target sequence:

(none)