Construct: shRNA TRCN0000256331

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000265.4-558s21c1
DNA Barcode:
CCATTGCCAACTACGAGAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NCF1 (653361)
Gene Description:
neutrophil cytosolic factor 1
Transcript:
RefSeq NM_000265.4 (NON-CURRENT)
Match location:
Position 558 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256331)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGCCAACTACGAGAAGA
Hairpin Sequence:
5'-CCGG-CCATTGCCAACTACGAGAAGA-CTCGAG-TCTTCTCGTAGTTGGCAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGCCAACTACGAGAAGACTCGAGTCTTCTCGTAGTTGGCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGCCAACTACGAGAAGACTCGAGTCTTCTCGTAGTTGGCAATGG-3'

Other clones with same target sequence:

(none)