Construct: shRNA TRCN0000256364

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004523.3-3979s21c1
DNA Barcode:
TGCAATGTAAATACGTATTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIF11 (3832)
Gene Description:
kinesin family member 11
Transcript:
RefSeq NM_004523.3 (NON-CURRENT)
Match location:
Position 3979 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256364)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCAATGTAAATACGTATTTC
Hairpin Sequence:
5'-CCGG-TGCAATGTAAATACGTATTTC-CTCGAG-GAAATACGTATTTACATTGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCAATGTAAATACGTATTTCCTCGAGGAAATACGTATTTACATTGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCAATGTAAATACGTATTTCCTCGAGGAAATACGTATTTACATTGCA-3'

Other clones with same target sequence:

(none)