Construct: shRNA TRCN0000256374

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001136106.2-684s21c1
DNA Barcode:
TTCACACGGTCTCCATGGACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BEAN1 (146227)
Gene Description:
brain expressed associated with NEDD4 1
Transcript:
RefSeq NM_001136106.2 (NON-CURRENT)
Match location:
Position 684 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256374)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCACACGGTCTCCATGGACA
Hairpin Sequence:
5'-CCGG-TTCACACGGTCTCCATGGACA-CTCGAG-TGTCCATGGAGACCGTGTGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCACACGGTCTCCATGGACACTCGAGTGTCCATGGAGACCGTGTGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCACACGGTCTCCATGGACACTCGAGTGTCCATGGAGACCGTGTGAA-3'

Other clones with same target sequence:

(none)