Construct: shRNA TRCN0000256393

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001111287.1-420s21c1
DNA Barcode:
TGGTAGCCGTGCATCTGTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Orai2-ps (100043803)
Gene Description:
ORAI calcium release-activated calcium modulator 2, pseudogene
Transcript:
RefSeq NM_001111287.1 (NON-CURRENT)
Match location:
Position 420 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256393)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTAGCCGTGCATCTGTTTG
Hairpin Sequence:
5'-CCGG-TGGTAGCCGTGCATCTGTTTG-CTCGAG-CAAACAGATGCACGGCTACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTAGCCGTGCATCTGTTTGCTCGAGCAAACAGATGCACGGCTACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTAGCCGTGCATCTGTTTGCTCGAGCAAACAGATGCACGGCTACCA-3'

Other clones with same target sequence:

(none)