Construct: shRNA TRCN0000256525

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001079516.1-135s21c1
DNA Barcode:
CTAAGGCTATTGCTACGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASAH2B (653308)
Gene Description:
N-acylsphingosine amidohydrolase 2B
Transcript:
RefSeq NM_001079516.1 (NON-CURRENT)
Match location:
Position 135 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256525)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAAGGCTATTGCTACGTATT
Hairpin Sequence:
5'-CCGG-CTAAGGCTATTGCTACGTATT-CTCGAG-AATACGTAGCAATAGCCTTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAAGGCTATTGCTACGTATTCTCGAGAATACGTAGCAATAGCCTTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAAGGCTATTGCTACGTATTCTCGAGAATACGTAGCAATAGCCTTAG-3'

Other clones with same target sequence:

(none)