Construct: shRNA TRCN0000256538

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001127388.1-478s21c1
DNA Barcode:
AGGGTCCAGATTTGGTTTCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DUX4L6 (653544)
Gene Description:
double homeobox 4 like 6 (pseudogene)
Transcript:
RefSeq NM_001127388.1 (NON-CURRENT)
Match location:
Position 478 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256538)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGGTCCAGATTTGGTTTCAG
Hairpin Sequence:
5'-CCGG-AGGGTCCAGATTTGGTTTCAG-CTCGAG-CTGAAACCAAATCTGGACCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGGTCCAGATTTGGTTTCAGCTCGAGCTGAAACCAAATCTGGACCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGGTCCAGATTTGGTTTCAGCTCGAGCTGAAACCAAATCTGGACCCT-3'

Other clones with same target sequence:

(none)