Construct: shRNA TRCN0000256541

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001127388.1-631s21c1
DNA Barcode:
TTTGAGAAGGATCGCTTTCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DUX4L6 (653544)
Gene Description:
double homeobox 4 like 6 (pseudogene)
Transcript:
RefSeq NM_001127388.1 (NON-CURRENT)
Match location:
Position 631 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256541)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTGAGAAGGATCGCTTTCCA
Hairpin Sequence:
5'-CCGG-TTTGAGAAGGATCGCTTTCCA-CTCGAG-TGGAAAGCGATCCTTCTCAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTGAGAAGGATCGCTTTCCACTCGAGTGGAAAGCGATCCTTCTCAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTGAGAAGGATCGCTTTCCACTCGAGTGGAAAGCGATCCTTCTCAAA-3'

Other clones with same target sequence:

(none)