Construct: shRNA TRCN0000256545

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014855.1-153s21c1
DNA Barcode:
TTCTGTTCCCGGATCTGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP5Z1 (9907)
Gene Description:
adaptor related protein complex 5 subunit zeta 1
Transcript:
RefSeq NM_014855.1 (NON-CURRENT)
Match location:
Position 153 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256545)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCTGTTCCCGGATCTGTAAA
Hairpin Sequence:
5'-CCGG-TTCTGTTCCCGGATCTGTAAA-CTCGAG-TTTACAGATCCGGGAACAGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCTGTTCCCGGATCTGTAAACTCGAGTTTACAGATCCGGGAACAGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCTGTTCCCGGATCTGTAAACTCGAGTTTACAGATCCGGGAACAGAA-3'

Other clones with same target sequence:

(none)