Construct: shRNA TRCN0000256563
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001039029.1-1155s21c1
- DNA Barcode:
- GTAACCTGCGTGAGTTCAAAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LRTM2 (654429)
- Gene Description:
- leucine rich repeats and transmembrane domains 2
- Transcript:
- RefSeq NM_001039029.1 (NON-CURRENT)
- Match location:
- Position 1155 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000256563)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GTAACCTGCGTGAGTTCAAAC
- Hairpin Sequence:
-
5'-CCGG-GTAACCTGCGTGAGTTCAAAC-CTCGAG-GTTTGAACTCACGCAGGTTAC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGTAACCTGCGTGAGTTCAAACCTCGAGGTTTGAACTCACGCAGGTTACTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGTAACCTGCGTGAGTTCAAACCTCGAGGTTTGAACTCACGCAGGTTAC-3'
Other clones with same target sequence:
(none)
