Construct: shRNA TRCN0000256592

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172207.2-837s21c1
DNA Barcode:
TGCAGAACCAGGCCCAGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMKK1 (84254)
Gene Description:
calcium/calmodulin dependent protein kinase kinase 1
Transcript:
RefSeq NM_172207.2 (CURRENT)
Match location:
Position 837 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256592)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCAGAACCAGGCCCAGAATA
Hairpin Sequence:
5'-CCGG-TGCAGAACCAGGCCCAGAATA-CTCGAG-TATTCTGGGCCTGGTTCTGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCAGAACCAGGCCCAGAATACTCGAGTATTCTGGGCCTGGTTCTGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCAGAACCAGGCCCAGAATACTCGAGTATTCTGGGCCTGGTTCTGCA-3'

Other clones with same target sequence:

(none)