Construct: shRNA TRCN0000256621

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006023.1-964s21c1
DNA Barcode:
AGACTTTGTGTTCGATATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC123 (8872)
Gene Description:
cell division cycle 123
Transcript:
RefSeq NM_006023.1 (NON-CURRENT)
Match location:
Position 964 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256621)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACTTTGTGTTCGATATATA
Hairpin Sequence:
5'-CCGG-AGACTTTGTGTTCGATATATA-CTCGAG-TATATATCGAACACAAAGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACTTTGTGTTCGATATATACTCGAGTATATATCGAACACAAAGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACTTTGTGTTCGATATATACTCGAGTATATATCGAACACAAAGTCT-3'

Other clones with same target sequence:

  1. TRCN0000246989