Construct: shRNA TRCN0000256666

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177151.3-1073s21c1
DNA Barcode:
ACCTATGTTTATCCGTATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps13b (666173)
Gene Description:
vacuolar protein sorting 13B
Transcript:
RefSeq NM_177151.3 (CURRENT)
Match location:
Position 1073 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256666)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTATGTTTATCCGTATAAT
Hairpin Sequence:
5'-CCGG-ACCTATGTTTATCCGTATAAT-CTCGAG-ATTATACGGATAAACATAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTATGTTTATCCGTATAATCTCGAGATTATACGGATAAACATAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTATGTTTATCCGTATAATCTCGAGATTATACGGATAAACATAGGT-3'

Other clones with same target sequence:

(none)