Construct: shRNA TRCN0000256709

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026168.3-311s21c1
DNA Barcode:
TGCGAATCAACATAGACATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ergic2 (67456)
Gene Description:
ERGIC and golgi 2
Transcript:
RefSeq NM_026168.3 (NON-CURRENT)
Match location:
Position 311 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256709)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCGAATCAACATAGACATTA
Hairpin Sequence:
5'-CCGG-TGCGAATCAACATAGACATTA-CTCGAG-TAATGTCTATGTTGATTCGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCGAATCAACATAGACATTACTCGAGTAATGTCTATGTTGATTCGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCGAATCAACATAGACATTACTCGAGTAATGTCTATGTTGATTCGCA-3'

Other clones with same target sequence:

(none)