Construct: shRNA TRCN0000256742

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001037866.1-607s21c1
DNA Barcode:
AGTCAATGAAAGGATTCTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RGPD6 (729540)
Gene Description:
RANBP2 like and GRIP domain containing 6
Transcript:
RefSeq NM_001037866.1 (CURRENT)
Match location:
Position 607 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256742)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCAATGAAAGGATTCTATT
Hairpin Sequence:
5'-CCGG-AGTCAATGAAAGGATTCTATT-CTCGAG-AATAGAATCCTTTCATTGACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCAATGAAAGGATTCTATTCTCGAGAATAGAATCCTTTCATTGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCAATGAAAGGATTCTATTCTCGAGAATAGAATCCTTTCATTGACT-3'

Other clones with same target sequence:

(none)